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Journal Article

Association of Procalcitonin Concentrations with Pathogenic Microorganisms

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Min Gu, Hua-Guo Xu
Clinical Chemistry, hvaa188, https://doi.org/10.1093/clinchem/hvaa188
Published: 25 August 2020
Journal Article

Correctly Interpreting SARS-CoV-2 Serologic Assays

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Stanley J Naides
Clinical Chemistry, hvaa165, https://doi.org/10.1093/clinchem/hvaa165
Published: 23 August 2020
Journal Article

Wiping the Slate Clean—Assessing Clinical Laboratory Contamination Risk

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Jonathan R Genzen
Clinical Chemistry, hvaa161, https://doi.org/10.1093/clinchem/hvaa161
Published: 23 August 2020
Journal Article

Routine laboratory blood tests predict SARS-CoV-2 infection using machine learning

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He S Yang, Yu Hou, Ljiljana V Vasovic, Peter Steel, Amy Chadburn ...
Clinical Chemistry, hvaa200, https://doi.org/10.1093/clinchem/hvaa200
Published: 21 August 2020
Journal Article

Ultrasensitive DNA Immune Repertoire Sequencing Using Unique Molecular Identifiers

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Gustav Johansson, Melita Kaltak, Cristiana Rîmniceanu, Avadhesh K Singh, Jan Lycke ...
Clinical Chemistry, hvaa159, https://doi.org/10.1093/clinchem/hvaa159
Published: 20 August 2020
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Immune repertoire sequencing of γδ T cells. a) Number of productive TRD mol...

Published: 20 August 2020
Fig. 3. Immune repertoire sequencing of γδ T cells. a) Number of productive TRD molecules for each assay, versus starting amount of γδ T-cell DNA. The linear correlation of each assay is shown, n = 3. b) Correction of molecule quantification using UMIs. Relative frequencies of clonotypes using raw sequencin
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Comparison of immune repertoire sequencing of enriched and non-enriched γδ ...

Published: 20 August 2020
Fig. 5. Comparison of immune repertoire sequencing of enriched and non-enriched γδ T cells from PBMCs. Peripheral blood mononuclear cells (PBMCs) from one individual were split in half: one sample was enriched for γδ T cells, while the other was analyzed directly without cell enrichment. a) Each point is a
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Illustration of δ T-cell receptor repertoire sequencing. a) Schematic overv...

Published: 20 August 2020
Fig. 1. Illustration of δ T-cell receptor repertoire sequencing. a) Schematic overview of the unrearranged TRD locus, arrows show transcription direction. b) SiMSen-Seq consists of two rounds of PCR. In the first barcoding PCR, target primers bind to the V and J genes, generating specific PCR products with
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Performance of 32-plex assay targeting TRD sequences. a) Dynamic range of 3...

Published: 20 August 2020
Fig. 2. Performance of 32-plex assay targeting TRD sequences. a) Dynamic range of 32-plex assay. Quantitative PCR on barcoded synthetic gBlock molecules, ranging from 2 · 10 ^ 7 to 20 molecules per standard with 10-fold dilution steps. Cycle of quantification value (Cq-value) is shown, n = 3
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The immune repertoire of TRD in healthy individuals. a) The number of produ...

Published: 20 August 2020
Fig. 4. The immune repertoire of TRD in healthy individuals. a) The number of productive TRD molecules detected by sequencing compared with the amount of γδ T-cell DNA used, assuming 139 productive molecules per nanogram DNA. b) Treemapping of all clonotypes across 10 healthy individuals. Each square repres
Journal Article

Throughput Considerations for a Sample-Multiplexed LC-MS/MS Assay: Is the Ability to Double the Injection Throughput Always a Time Saver?

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David A Wells
Clinical Chemistry, hvaa171, https://doi.org/10.1093/clinchem/hvaa171
Published: 18 August 2020
Journal Article

Use of “Coldspot” Regions in Variant Classification

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Steven M Harrison, Birgit Funke
Clinical Chemistry, hvaa133, https://doi.org/10.1093/clinchem/hvaa133
Published: 16 August 2020
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Mass spectrum of peptide a) MVLYTLRAPRSPK generated by Lys-C from proBNP in...

Published: 14 August 2020
Fig. 2. Mass spectrum of peptide a) MVLYTLRAPRSPK generated by Lys-C from proBNP in an 18 O buffer; Blue peaks reflect single 18 O labeled 12 C and 13 C peaks of the peptide, whereas red peaks reflect nonlabeled signals; The abundance of the nonlabeled peptide matches the manufacturer’s claims of 16 O
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Relative distribution of nonglycosylated N-terminally truncated (NT-) proBN...

Published: 14 August 2020
Fig. 3. Relative distribution of nonglycosylated N-terminally truncated (NT-) proBNP forms circulating in 8 HF patients; depicted is the mean value and range of 3 replicates; missing values indicate no signal detected.
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Overview of identified peptides corresponding to in vivo a) N-terminally tr...

Published: 14 August 2020
Fig. 6. Overview of identified peptides corresponding to in vivo a) N-terminally truncated molecular forms described in Fig. 3 and b) C-terminally truncated molecular forms, NT-proBNP or proBNP described in Fig. 4 .
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Schematic overview of identified circulating molecular forms of proBNP and ...

Published: 14 August 2020
Fig. 1. Schematic overview of identified circulating molecular forms of proBNP and NT-proBNP in HF patients and controls. Also shown is the current understanding of proBNP processing by furin (red arrow) and corin (green arrows) as well as O-glycosylation and N- and C-terminal degradation of (NT-) proBNP; b
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Relative distribution of nonglycosylated C-terminally truncated (NT-) proBN...

Published: 14 August 2020
Fig. 4. Relative distribution of nonglycosylated C-terminally truncated (NT-) proBNP forms, NT-proBNP and proBNP circulating in 8 HF patients; depicted is the mean value and range of three replicates.
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Relative distribution of A) nonglycosylated N-terminally truncated (NT-) pr...

Published: 14 August 2020
Fig. 5. Relative distribution of A) nonglycosylated N-terminally truncated (NT-) proBNP forms circulating in 2 healthy subjects, and B) nonglycosylated C-terminally truncated (NT-) proBNP forms circulating in 2 healthy subjects; missing value indicates no signal detected.
Journal Article

Exposing the High Heterogeneity of Circulating Pro B-Type Natriuretic Peptide Fragments in Healthy Individuals and Heart Failure Patients

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Benno Amplatz, Bettina Sarg, Klaus Faserl, Angelika Hammerer-Lercher, Johannes Mair ...
Clinical Chemistry, hvaa130, https://doi.org/10.1093/clinchem/hvaa130
Published: 14 August 2020
Journal Article

Evaluation of the Risk of Laboratory Microbial Contamination during Routine Testing in Automated Clinical Chemistry and Microbiology Laboratories

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Christopher W Farnsworth, Meghan A Wallace, Albert Liu, Ann M Gronowski, Carey-Ann D Burnham ...
Clinical Chemistry, hvaa128, https://doi.org/10.1093/clinchem/hvaa128
Published: 13 August 2020