Allicin induces p53-mediated autophagy in Hep G2 human liver cancer cells
- PMID: 22860996
- DOI: 10.1021/jf301298y
Allicin induces p53-mediated autophagy in Hep G2 human liver cancer cells
Abstract
Garlic has been used throughout history for both culinary and medicinal purpose. Allicin is a major component of crushed garlic. Although it is sensitive to heat and light and easily metabolized into various compounds such as diallyl disulfide, diallyl trisulfide, and diallyl sulfide, allicin is still a major bioactive compound of crushed garlic. The mortality of hepatocellular carcinoma is quite high and ranks among the top 10 cancer-related deaths in Taiwan. Although numerous studies have shown the cancer-preventive properties of garlic and its components, there is no study on the effect of allicin on the growth of human liver cancer cells. In this study, we focused on allicin-induced autophagic cell death in human liver cancer Hep G2 cells. Our results indicated that allicin induced p53-mediated autophagy and inhibited the viability of human hepatocellular carcinoma cell lines. Using Western blotting, we observed that allicin decreased the level of cytoplasmic p53, the PI3K/mTOR signaling pathway, and the level of Bcl-2 and increased the expression of AMPK/TSC2 and Beclin-1 signaling pathways in Hep G2 cells. In addition, the colocalization of LC3-II with MitoTracker-Red (labeling mitochondria), resulting in allicin-induced degradation of mitochondria, could be observed by confocal laser microscopy. In conclusion, allicin of garlic shows great potential as a novel chemopreventive agent for the prevention of liver cancer.
Similar articles
-
Allicin induces anti-human liver cancer cells through the p53 gene modulating apoptosis and autophagy.J Agric Food Chem. 2013 Oct 16;61(41):9839-48. doi: 10.1021/jf403241s. Epub 2013 Oct 7. J Agric Food Chem. 2013. PMID: 24059278
-
Beclin 1-mediated autophagy in hepatocellular carcinoma cells: implication in anticancer efficiency of oroxylin A via inhibition of mTOR signaling.Cell Signal. 2012 Aug;24(8):1722-32. doi: 10.1016/j.cellsig.2012.04.009. Epub 2012 Apr 25. Cell Signal. 2012. PMID: 22560876
-
Induction of α-phellandrene on autophagy in human liver tumor cells.Am J Chin Med. 2015;43(1):121-36. doi: 10.1142/S0192415X15500081. Epub 2015 Feb 4. Am J Chin Med. 2015. PMID: 25649747
-
Garlic and its Active Compounds: A Potential Candidate in The Prevention of Cancer by Modulating Various Cell Signalling Pathways.Anticancer Agents Med Chem. 2019;19(11):1314-1324. doi: 10.2174/1871520619666190409100955. Anticancer Agents Med Chem. 2019. PMID: 30963982 Review.
-
Targeting autophagic pathways for cancer drug discovery.Chin J Cancer. 2013 Mar;32(3):113-20. doi: 10.5732/cjc.012.10010. Epub 2012 Jul 26. Chin J Cancer. 2013. PMID: 22835386 Free PMC article. Review.
Cited by 16 articles
-
The Synergistic Antitumor Effect of 5-Fluorouracil Combined with Allicin against Lung and Colorectal Carcinoma Cells.Molecules. 2020 Apr 22;25(8):1947. doi: 10.3390/molecules25081947. Molecules. 2020. PMID: 32331446 Free PMC article.
-
Overexpression miR-486-3p Promoted by Allicin Enhances Temozolomide Sensitivity in Glioblastoma Via Targeting MGMT.Neuromolecular Med. 2020 Sep;22(3):359-369. doi: 10.1007/s12017-020-08592-5. Epub 2020 Feb 21. Neuromolecular Med. 2020. PMID: 32086739 Free PMC article.
-
The Role of Herbal Bioactive Components in Mitochondria Function and Cancer Therapy.Evid Based Complement Alternat Med. 2019 Jun 12;2019:3868354. doi: 10.1155/2019/3868354. eCollection 2019. Evid Based Complement Alternat Med. 2019. PMID: 31308852 Free PMC article. Review.
-
Impact of Diet and Nutrition on Cancer Hallmarks.J Cancer Prev Curr Res. 2017;7(4):240. doi: 10.15406/jcpcr.2017.07.00240. Epub 2017 Feb 17. J Cancer Prev Curr Res. 2017. PMID: 30581989 Free PMC article.
-
Diallyl trisulfide exerts cardioprotection against myocardial ischemia-reperfusion injury in diabetic state, role of AMPK-mediated AKT/GSK-3β/HIF-1α activation.Oncotarget. 2017 Aug 24;8(43):74791-74805. doi: 10.18632/oncotarget.20422. eCollection 2017 Sep 26. Oncotarget. 2017. PMID: 29088824 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
-
Full Text Sources
-
Other Literature Sources
-
Medical
-
Research Materials
-
Miscellaneous