Cinnamaldehyde induces apoptosis by ROS-mediated mitochondrial permeability transition in human promyelocytic leukemia HL-60 cells
- PMID: 12860272
- DOI: 10.1016/s0304-3835(03)00238-6
Cinnamaldehyde induces apoptosis by ROS-mediated mitochondrial permeability transition in human promyelocytic leukemia HL-60 cells
Abstract
Cinnamaldehyde is an active compound isolated from the stem bark of Cinnamomum cassia, a traditional oriental medicinal herb, which has been shown to inhibit tumor cell proliferation. In this study, we investigated the effects of cinnamaldehyde on the cytotoxicity, induction of apoptosis and the putative pathways of its actions in human promyelocytic leukemia cells. Using apoptosis analysis, measurement of reactive oxygen species (ROS), and assessment of mitochondrial membrane potentials (DeltaPsim), we show that cinnamaldehyde is a potent inducer of apoptosis and that it transduces the apoptotic signal via ROS generation, thereby inducing mitochondrial permeability transition (MPT) and cytochrome c release to the cytosol. ROS production, mitochondrial alteration, and subsequent apoptotic cell death in cinnamaldehyde-treated cells were blocked by the antioxidant N-acetylcystein. Taken together, our data indicate that cinnamaldehyde induces the ROS-mediated mitochondrial permeability transition and resultant cytochrome c release. This is the first report on the mechanism of the anticancer effect of cinnamaldehyde.
Similar articles
-
Costunolide induces apoptosis by ROS-mediated mitochondrial permeability transition and cytochrome C release.Biol Pharm Bull. 2001 Mar;24(3):303-6. doi: 10.1248/bpb.24.303. Biol Pharm Bull. 2001. PMID: 11256490
-
Effects of vitamin E on the cinnamaldehyde-induced apoptotic mechanism in human PLC/PRF/5 cells.Clin Exp Pharmacol Physiol. 2004 Nov;31(11):770-6. doi: 10.1111/j.1440-1681.2004.04091.x. Clin Exp Pharmacol Physiol. 2004. PMID: 15566391
-
2,3,5-tris(Glutathion-S-yl)hydroquinone (TGHQ)-mediated apoptosis of human promyelocytic leukemia cells is preceded by mitochondrial cytochrome c release in the absence of a decrease in the mitochondrial membrane potential.Toxicol Sci. 2005 Jul;86(1):92-100. doi: 10.1093/toxsci/kfi165. Epub 2005 Mar 30. Toxicol Sci. 2005. PMID: 15800030
-
Bcr-Abl exerts its antiapoptotic effect against diverse apoptotic stimuli through blockage of mitochondrial release of cytochrome C and activation of caspase-3.Blood. 1998 Mar 1;91(5):1700-5. Blood. 1998. PMID: 9473236
-
Interaction of biologically active amines with mitochondria and their role in the mitochondrial-mediated pathway of apoptosis.Curr Med Chem. 2004 Sep;11(17):2349-74. doi: 10.2174/0929867043364559. Curr Med Chem. 2004. PMID: 15379717 Review.
Cited by 42 articles
-
Oxidative resistance of leukemic stem cells and oxidative damage to hematopoietic stem cells under pro-oxidative therapy.Cell Death Dis. 2020 Apr 27;11(4):291. doi: 10.1038/s41419-020-2488-y. Cell Death Dis. 2020. PMID: 32341354 Free PMC article. Review.
-
Evaluation of the cytotoxic and apoptogenic effects of cinnamaldehyde on U87MG cells alone and in combination with doxorubicin.Res Pharm Sci. 2020 Feb 20;15(1):26-35. doi: 10.4103/1735-5362.278712. eCollection 2020 Feb. Res Pharm Sci. 2020. PMID: 32180814 Free PMC article.
-
Protective effects of 2-methoxycinnamaldehyde an active ingredients of Cinnamomum cassia on warm hepatic ischemia reperfusion injury in rat model.Iran J Basic Med Sci. 2019 Dec;22(12):1400-1407. doi: 10.22038/IJBMS.2019.13987. Iran J Basic Med Sci. 2019. PMID: 32133057 Free PMC article.
-
Potent USP10/13 antagonist spautin-1 suppresses melanoma growth via ROS-mediated DNA damage and exhibits synergy with cisplatin.J Cell Mol Med. 2020 Apr;24(7):4324-4340. doi: 10.1111/jcmm.15093. Epub 2020 Mar 4. J Cell Mol Med. 2020. PMID: 32129945 Free PMC article.
-
Cinnamic Acid Conjugates in the Rescuing and Repurposing of Classical Antimalarial Drugs.Molecules. 2019 Dec 24;25(1):66. doi: 10.3390/molecules25010066. Molecules. 2019. PMID: 31878190 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
-
Full Text Sources
-
Other Literature Sources
-
Research Materials