Dietary Sources of Lutein and Zeaxanthin Carotenoids and Their Role in Eye Health
El-Sayed M. Abdel-Aal
1 Guelph Food Research Centre, Agriculture and Agri-Food Canada, Guelph, ON N1G 5C9, Canada; E-Mail: firstname.lastname@example.org
1 Guelph Food Research Centre, Agriculture and Agri-Food Canada, Guelph, ON N1G 5C9, Canada; E-Mail: email@example.com
2 Consultant, Toronto, ON M3M 2E9, Canada; E-Mail: moc.liamg@0002reehazk
The eye is a major sensory organ that requires special care for a healthy and productive lifestyle. Numerous studies have identified lutein and zeaxanthin to be essential components for eye health. Lutein and zeaxanthin are carotenoid pigments that impart yellow or orange color to various common foods such as cantaloupe, pasta, corn, carrots, orange/yellow peppers, fish, salmon and eggs. Their role in human health, in particular the health of the eye, is well established from epidemiological, clinical and interventional studies. They constitute the main pigments found in the yellow spot of the human retina which protect the macula from damage by blue light, improve visual acuity and scavenge harmful reactive oxygen species. They have also been linked with reduced risk of age-related macular degeneration (AMD) and cataracts. Research over the past decade has focused on the development of carotenoid-rich foods to boost their intake especially in the elderly population. The aim of this article is to review recent scientific evidences supporting the benefits of lutein and zexanthin in preventing the onset of two major age-related eye diseases with diets rich in these carotenoids. The review also lists major dietary sources of lutein and zeaxanthin and refers to newly developed foods, daily intake, bioavailability and physiological effects in relation to eye health. Examples of the newly developed high-lutein functional foods are also underlined.
Nutrition plays a vital role in human health with no exception to the eye. Healthy eyes provide good vision, which is essential for an enjoyable and productive lifestyle. There is a growing global concern about eye health related issues. To address these issues the World Health Organization (WHO) report on visual impairment in 2010 identifies the principal causes of visual impairment as follows: uncorrected refractive errors (43%), cataracts (33%), glaucoma (2%), age-related macular degeneration (AMD) (1%), diabetic retinopathy (1%) and about 18% are of undetermined nature . The report also lists the three major causes of blindness as cataract (51%), glaucoma (8%), AMD (5%), diabetic retinopathy (1%) and undetermined causes (21%). Cataract is the principal cause of blindness among people over 40 years of age predominantly in developing countries due to improper nutrition (e.g., lack of carotenoids in diet), infectious diseases . AMD, on the other hand, is the leading cause of legal blindness with limited treatment options in people over 65 years of age in industrial countries, and costs many billions of dollars worldwide . There are two types of AMD—dry (atrophic) and wet (neovascular or exudative). In most cases AMD starts as “Dry”, which then progresses slowly in approximately 20% cases to “Wet” stage. There is no known treatment for dry AMD. The wet AMD is responsible for almost 90% of the severe cases of blindness . The prevalence of AMD is estimated to be a one-third increase in UK  and a 50% increase in USA by 2020 . A similar estimate is also reported for Australia . Both diseases are expected to sharply increase in the elderly population within the next 10–15 years. The Vision 2020, a global initiative for the elimination of avoidable blindness in partnership between WHO and International Agency for the Prevention of Blindness [IAPB] projects blindness due to cataracts alone in elderly to reach 40 million globally by 2025 . It is clear that AMD and cataract incidences will continue to rise substantially in the coming years causing a great impact on health care. This requires global collective efforts to develop strategies for the prevention of the two most important common age-related diseases with appropriate diets/supplements. Several high-lutein functional foods that would be useful in developing such preventive strategies are discussed in the current article.
Oxidative stress, aging and smoking are known to cause cataract and AMD [8,9]. A typical US diet contains 1–3 mg/day of lutein and zeaxanthin, while ~6 mg/day have been related to decrease risk of AMD . Lutein and zeaxanthin have been associated with reduced risk of cataract development and AMD . A longitudinal study has shown that plasma zeaxanthin reduces the risk of cataract . Hence lutein and zeaxanthin, both potent antioxidants, are very important to retard the onset of both cataract and AMD . Additional sources of lutein and zeaxanthin either in the food form or dietary supplements would enhance their intake on regular basis. There are also reports that do not support the protective role of dietary lutein and zeaxanthin against cataracts  and AMD . Recently an article that traces the modern history of lutein and zeaxanthin in health and diseases of retina identified four areas for further investigation: (i) ultra-structural localization of xanthophyll affected proteins in the retina, (ii) genetic analysis-genotyping efforts, (iii) model system for the metabolism of lutein and zeaxanthin and (iv) integrated system based approaches to trace the fate of lutein and zeaxanthin, its precursor(s) and metabolites .
This review article mainly focuses on lutein and zeaxanthin carotenoids in terms of their sources of common and newly developed foods, daily intake, bioavailability and physiological effects in relation to eye health. Examples of the newly developed high-lutein functional foods are underlined as well.
2. Lutein and Zeaxanthin Carotenoids
Lutein and zeaxanthin are relatively polar carotenoid pigments found at high levels in parsley, spinach, kale, egg yolk and lutein-fortified foods. They have demonstrated several beneficial health effects due to their ability to act as scavengers for reactive oxygen species and to bind with physiological proteins in humans . In general, carotenoids are tetra-terpenoid having 40 carbon skeleton made up of 8 isoprene units and comprise of two classes, namely carotenes (purely unsaturated hydrocarbons) and carotenoids with oxygen atoms which are referred to as oxygenated carotenoids or xanthophyll carotenoids. The macular carotenoids are dietary lutein and zeaxanthin, and their conversion isomer meso-zeaxanthin, which are non-provitamin A carotenoids, (i.e., it cannot be converted into vitamin A). Important members of oxygenated carotenoids are lutein, zeaxanthin, β-cryptoxanthin, capsanthin, astaxanthin, and fucoxanthin. Figure 1 lists the chemical structure of the macular pigments found in the retina. It is estimated that about 90% of the total carotenoids in North American diets consist of lycopene, β-carotene, α-carotene, lutein, β-cryptoxanthin and zeaxanthin . In nature more than 600 carotenoids have been isolated and characterized, yet only about 40 carotenoids have been detected in human milk, serum and tissues. Percentage of main carotenoids inhuman serum is lutein (20%), lycopene (20%), β-carotene (10%); β-cryptoxanthin (8%), α-carotene (6%) and zeaxanthin (3%) [18,19]. Lutein and zeaxanthin are the main dietary carotenoids found in human retina  and they protect the macula from damage by blue light, improve visual acuity and scavenge harmful reactive oxygen. Lutein and zeaxanthin along with their common metabolite meso-zeaxanthin, commonly referred to as macular pigments (MP) . The ratio between lutein, zeaxanthin and meso-zeaxanthin changes as the eccentricity moves away from fovea [21,22,23]. Although lutein and zeaxanthin were also detected in prenatal eyes, they did not form visible yellow spot. No age-related (between the ages of 3 and 95 years) differences were observed in the quantity of lutein and zeaxanthin . But, the ratio of lutein to zeaxanthin differed between infants and adults. In infants, lutein predominates over zeaxanthin in fovea, and the opposite is true after 3 years of age [21,24]. Structurally the difference between lutein and zeaxanthin is in the type of ionone ring, lutein contains a β-ionone ring and a ε-ionone ring, whereas zeaxanthin has two β-ionone rings. Lutein and zeaxanthin are isomers, but not stereoisomers, which differ in the location of a double bond unsaturation in the end ring (Figure 1). Lutein can exist in possible eight stereoisomeric forms because of three chiral centers, but in nature it exists mainly in Z (cis)-form (R,R,R). Zeaxanthin, on the other hand, has two chiral centers but, because of symmetry exists only in 3-stereoisomeric forms (R,R), (S,S) and (R,S-meso) (Figure 1).
2.2. Dietary Sources
Lutein and zeaxanthin are the most common xanthophylls in green leafy vegetables (e.g., kale, spinach, broccoli, peas and lettuce) and egg yolks  (Table 1). They are also found at relatively high levels in einkorn, Khorasan and durum wheat and corn and their food products [26,27,28,29] (Table 1). The ratio of lutein and zeaxanthin in green vegetables has been reported to range between 12 to 63, highest being in kale, while in yellow-orange fruits and vegetable this ratio ranges between 0.1 and 1.4 . They also quantified small amounts of lutein and zeaxanthin in breads prepared from modern wheat varieties, Pioneer and Catoctin, while breads prepared from green-harvested wheat, Freekeh, an ancient grain, contained considerably large amounts of lutein and zeaxanthin compared to the North American breads. Lutein to zeaxanthin ratio followed the order Pioneer > Catochtin > Freekeh . Chicken egg yolk is deemed a better source of lutein and zeaxanthin compared to fruits and vegetables because of its increased bioavailability due to the high fat content in eggs [31,32]. The concentrations of lutein and zeaxanthin in chicken egg yolk are 292 ± 117 µg/yolk and 213 ± 85 µg/yolk (average weight of yolk is about 17–19 g), respectively and are likely dependent on the type of feed, found mainly in on-esterified form with minute amounts of lycopene and β-carotene . It is not surprising that egg noodle had almost 6 times more xanthophyll carotenoids than lasagne . Astaxanthin and fucoxanthin are abundant in green and brown algae, respectively, which are eaten by fish. Capsanthin is found mainly in pepper. β-Cryptoxanthin is a pro-vitamin A and found in many fruits and vegetables, but mainly in corn, oranges, peaches, papaya, watermelon, and egg yolk [34,35].
|Green pepper a||8.8||-|
|Red pepper a||2.5–85.1||5.9–13.5|
|Egg yolk a||3.84–13.2||-|
|High lutein bread b||36.7||3.3|
|High lutein cookie b||21.3||2.9|
|High lutein muffin b||26.1||3.7|
|Corn tortilla c||72.5||105.3|
|Corn chips c||61.1||92.5|
|Einkorn wheat d||7.4||0.9|
|Khorasan wheat d||5.5||0.7|
|Durum wheat d||5.4||0.5|
In general carotenoids are very minor constituents in cereal grains except for einkorn and durum wheat and corn that contain relatively high levels of carotenoids or yellow pigments [27,29,36]. The common carotenoids in cereal grains are α and β-carotene, β-cryptoxanthin, lutein and zeaxanthin with lutein being the dominant carotenoid compound. In common wheat flour (low in carotenoids), the bran/gem fraction had 4-fold more lutein, 12-fold more zeaxanthin, and 2-fold more β-cryptoxanthin than the endosperm fractions . Higher amounts of lutein were found in durum, Kamut and Khorasan (5.4–5.8 µg/g) compared with common bread and pastry wheat (2.0–2.1 µg/g). Einkorn, on the other hand, had the highest concentration of all-trans-lutein, which is influenced by environmental growing conditions  and processing . Corn also contains exceptionally high levels of non-provitamin A carotenoids primarily lutein and zeaxanthin [29,38].
In order to exert and deliver their physiological effects carotenoids must be absorbed and transported into the blood stream. In general, carotenoids are lipophilic or hydrophobic which are soluble in fat and insoluble in aqueous media, the medium of human digestive system. Because of the hydroxyl groups, lutein and zeaxanthin are polar compounds compared with the hydrocarbon carotenoids (α-, β-carotene, and lycopene). Thus, a good understanding of carotenoid release, absorption, transportation and accumulation in eye is essential to evaluate the benefits.
The major factors that influence the absorption of carotenoids including lutein and zeaxanthin from food include (i) nature of the food matrix, e.g., in natural format, cooked or supplement, (ii) amount and nature of the dietary fat, which aids in the solubilisation of released carotenoids, (iii) phospholipids, (iv) dietary fiber, (v) nature of carotenoids [39,40,41]. The absorption of carotenoid released from food include several steps (i) dispersion in the gastric emulsion to be incorporated into lipid droplets, (ii) followed by transfer to mixed micelles involving bile salts, biliary phospholipids, dietary lipids and others. Solubilized carotenoids are then absorbed by the intestinal cell for transportation into blood system. These steps may include simple diffusion, uptake by micelles and receptor mediated and other transporter, as schematically presented by Nagao and colleagues [42,43]. The highest concentration of carotenoids in micelles (i.e., solubilisation), corresponds to greater absorption and transportation into plasma. In general, bioavailability of carotenoids is affected by a number of factors including food matrix, processing conditions and fat content [39,44], while the rate of bio-accessibility of carotenoids is greatly impacted by food matrix and processing. It was observed that the in vitro rate of lutein, zeaxanthin and β-cryptoxanthin transfer almost 100% from fruits (orange, kiwi, grapefruit and sweet potato) compared to between 19% and 38% from spinach and broccoli, respectively . The release of carotenoids from a food matrix followed by absorption is the determining factors for delivering the anticipated health benefits. Since carotenoids are found in a food matrix, they could be released prior to consumption by processing and heat treatment . In other words, the intestinal absorption and metabolic transformation determine the efficacies of carotenoids including transportation and accumulation of macula pigments (MP) in retina that leads to protection of retina, possible prevention and/or slowing the progress of blindness.
Dietary lutein and zeaxanthin and the metabolite meso-zeaxanthin are concentrated (~25% of the total carotenoids) in the macula region of healthy eye as a yellow spot, but considerably less in deceased eyes [47,48,49]. meso-Zeaxanthin a non-dietary carotenoid is not found in serum, but only in retina. It has been suggested that lutein and zeaxanthin are transported into retina in the same ratio as in plasma, and then transferred to macula where lutein is preferentially converted into meso-zeaxanthin [47,48,49,50,51]. These observations strongly suggest the importance of lutein, zeaxanthin and meso-zeaxanthin in the management of good eye health [51,52,53]. Several studies have shown that incidences of AMD can be reduced by consuming diets with high levels of lutein and zeaxanthin and supplementation by increasing their concentration in serum and parallel increase in macular pigment optical density (MPOD) [54,55,56,57,58]. For example, lutein supplementation over a 140-day period increased serum lutein level . Similarly, consuming increased spinach and kale in diet for a 4-week period increased the MPOD by 4%–5%. Recently, a systematic review and meta-analysis of several longitudinal studies have concluded that lutein and zeaxanthin affect positively in the case of late AMD but not early AMD . The early or dry AMD was defined by the presence of drusen pigment abnormalities in retina pigment epithelium (RPE) or both whereas the late or wet AMD includes neovascular AMD and geographic atrophy by the presence of choroidal neovascularization, detachment of RPE or geographic atrophy .
Concentration and nature of various carotenoids in eye (macula and retina) were established in early 1990. In the fovea, the carotenoid concentration approaches 1 mM, and the ratio of lutein to zeaxanthin to meso-zeaxanthin is 1:1:1 . The concentration of macular carotenoids declines over 100-fold just a few millimeters from the foveal center, and the composition ratio approaches 3:1:0 in the peripheral retina (around 21 mm) i.e., lutein concentration is considerably higher with no zeaxanthin in peripheral retina [47,50,60]. The exclusiveness (>80% of total carotenoids) of lutein and zeaxanthin in retina from among 40 carotenoids found in serum is unique and intriguing since the presence of about 20%–25% of total carotenoids in human plasma cannot account for by routine transportation systems [61,62]. In general, carotenoids are transported mainly by low-density lipoprotein (LDL, 55%), followed by high-density lipoprotein (HDL, 33%) and very low density lipoprotein (VLDL, 10% to 19%) and others . However, lutein and zeaxanthin are distributed equally in LDL and HDL, with more infinity towards HDL . These data suggest that lipoprotein profile of serum would play important role in transport and concentration of lutein and zeaxanthin in retina and MPOD. A study reported negative relationship between serum triglycerides and MP, and no relations between MPOD and HDL and LDL . The authors also observed lower than the normal serum lutein and zeaxanthin levels. On the other hand, another study found positive relationships between serum lutein and zeaxanthin with total cholesterol and HDL, inversely related to triacylglycerols, but none with LDL . A most recent cross-sectional study observed that serum lutein and zeaxanthin and lipoprotein concentrations are significantly related . The study concludes that changing lipoprotein concentrations may impact retinal lutein and zeaxanthin levels. Evidences for HDL primary role in the transport of lutein and zeaxanthin were provided when it was reported that feeding high lutein diet to the Wisconsin Hypoalpha Mutant Chicken deficient in HDL increased lutein concentration significantly in various tissues/organ except in retina . Egg, a rich source of lutein and zeaxanthin, is an integral part of the American diet, but with continued concerns for increasing serum lipids and lipoproteins concentrations. A randomized cross-over design study involving 33 men and women consuming 1 egg per day for 5 weeks reported increased serum lutein (26%), and zeaxanthin (38%), but serum concentrations of total cholesterol, LDL cholesterol, HDL cholesterol and triacylglycerols were not affected . Most recently it was reported that daily intake of 3 eggs for 12 weeks increased the lutein and zeaxanthin by 21% and 48%, respectively in 20 adults . Thus, egg yolk could be an important dietary source to improve lutein and zeaxanthin status for the prevention of cataracts and AMD in adults. Similarly, a separate study with simultaneous administration of large amounts of lutein, zeaxanthin and β-carotene affected their concentrations in plasma, tissues and retina . The retinal concentration of lutein and zeaxanthin increased 128% and 116% respectively, when fed diet with high lutein (27.2 mg/kg) and zeaxanthin (15.3 mg/kg) for 28 days compared to the control diet with lutein (5.2 mg/kg) and zeaxanthin (1.7 mg/kg). Further, it was observed that supplementation of β-carotene increased its value in plasma very little, and none in the other tissues and retina. However, high supplementation of β-carotene in conjunction with high lutein and zeaxanthin diet decreased lutein and zeaxanthin concentration in plasma, tissue and retina . The mechanism is not well understood. A recent explanatory study using a formulation consisting of all the three MP carotenoids (7.3 mg meso-zeaxanthin, 3.7 mg lutein and 0.8 mg zeaxanthin) over an 8 week period significantly increased the serum concentration of these carotenoids as well as the MPOD .
It is more likely that transportation and accumulation of lutein and zeaxanthin involve preferential and efficacious complex-binding with other circulating proteins. Such xanthophyll-lipoprotein complex(s) are then circulated in blood stream. It is interesting to note that blue colouration in lobster shell has been shown to be due to the complex-binding of carotenoid (astaxanthin) with a protein referred to as crustacyanin . It would, therefore, be reasonable to explore similar complex-binding protein(s) in humans and other vertebrates that may be responsible for transportation of lutein and zeaxanthin to the macula. Bhosale and co-workers  reported that Pi isoform of glutathione transferase protein (GSTPi) in the human macula has a greater interaction with zeaxanthin and meso-zeaxanthin compared with lutein which has a weaker interaction. Subsequently, these researchers also purified and identified a lutein-binding protein in the macula of human eye labeled as StARD3 (Steroidogmic regulatory domain), also known as MNL64 [74,75]. Another team investigated the mechanism for preferential uptake of xanthophylls by retina pigment epithelial (RPE) cells. They reported that RPE cells preferentially take up xanthophylls than other carotenes by SR-BI dependent-dependent process(s) to preferentially accumulate in the macula of retina . Overall, absorption and transportation of carotenoids in foods are complex multi-step processes.
2.4. Eye Health
Considerable research has been undertaken on global level to generate new knowledge to understand the causes, and define steps/processes to slow the onset of cataract and AMD through diets. Evidences show that lutein and zeaxanthin are important dietary carotenoids in preventing and reducing cataracts and AMD. A multi-center eye disease case-control study involving five ophthalmology centers in the US showed that a higher dietary intake of carotenoids, specifically lutein and zeaxanthin is associated with reduced AMD risk . Thus, our efforts will be limited to reviewing the existing literature on the role of dietary carotenoids in reducing the incidences of cataract and AMD. In general, the ageing processes cause biochemical, physiological and physical changes that are directly or indirectly responsible for the onset of many diseases including cataract and AMD. The pathogeneses of both cataract and AMD formation are not fully established. However, over the years research has identified major contributing factors for both diseases. Ageing (greater than 50 years of age) seems to be the major cause in both cases. In addition, other factors include exposure to ultraviolet light (up to around 380 nm) and blue light (400–500 nm high energy), oxidative stress due to access of oxygen radical species, environmental factors, and high polyunsaturated fatty acids responsible for AMD . High incidences of cataract have been linked to poverty and poor nutrition, and strict vegetarian diets lacking in antioxidants [78,79].
High macular pigment density (MPD), macular pigment optical density (MPOD) and macular pigment (MP) have been associated with reduced AMD. There are studies that have linked ethnicity to the development of AMD because of differences in the MPD, MPOD and MP distribution in retina among various races. For example, Wolf-Schnurrbusch and co-workers  recorded significantly higher MPD in African subjects compared to White non-Hispanic subjects (0.59 ± 0.14 DU versus 0.36 ± 0.13 DU). This provides scientific explanations for previously observed higher incidences of AMD in white non-Hispanic compared to African populations in East Baltimore, USA. Further, a reverse trend was observed for cataracts, i.e., its incidence is 4 times higher in Blacks than White non-Hispanic subjects . These observations have been supported by several other studies that have linked lutein and zeaxanthin concentrations to the reduction of cataracts in North Indian population , in older American women  and in Australian population . Recent studies also reported the prevalence of major eye diseases and showed that high plasma concentrations of lutein and zeaxanthin reduced the risk of age-related cataract in the elderly Finnish population by about 41% [85,86]. Studies involving older Europeans from Norway, Estonia, United Kingdom, France, Italy, Greece and Spain have reported high AMD prevalence (4% to 12%) depending on age . However, studies from China, India and Korea show a prevalence rate of around 4% [88,89,90]. The Beijing study  involved 4439 subjects over 40 years of age residing in rural and urban areas and the Korean studies had 10,449 subjects over 40 years . These studies suggest less prevalence of AMD in Asians than Caucasians. In addition, several epidemiological studies have found a close relationship between dietary carotenoids, more specifically the amount of lutein and zeaxanthin, and the incidences of AMD [91,92,93].
3. High-Lutein Functional Foods
As mentioned earlier a number of wheat species such as einkorn (ancient wheat) and durum (pasta wheat) and corn hold a potential for developing high-lutein staple foods. These cereals were identified as promising ingredients for the development of high-lutein functional foods based on their relatively higher levels of lutein compared with other wheat species such as spelt, soft and hard wheat [94,95]. Lutein content ranges from 5.4 to 7.4 µg/g in high-lutein wheat species and about 21.9 µg/g in corn. Lutein and zeaxanthin are the major carotenoids in corn milled fractions and account for about 70% of the total carotenoids . This makes corn a promising blending flour ingredient in the development of high-lutein functional foods.
Three wholegrain functional foods with high level of lutein (about 1 mg per 30 g serving) were developed and evaluated in terms of lutein stability during baking process , lutein digestibility in vitro using fasted and fed model , phenolic antioxidants , and antioxidant properties . The wholegrain bakery products include high-lutein flat bread, high-lutein cookie and high-lutein muffin. Lutein was found to drop significantly during baking process (28% to 64% loss) due to oxidation and isomerization. A number of cis-isomers were found in the three products with 13- and 13′-cis-lutein being the dominant cis-isomers. Due to the significant losses of lutein a fortification approach was used to boost lutein in the functional food products and to compensate for the losses of lutein occurred during processing and/or storage. Other approaches could also be used such as protection of lutein during processing or developing wheat and corn verities with higher lutein content than the existing ones. Despite the significant losses of lutein during processing, the developed fortified baked products still contain reasonable concentrations (up to 1 mg/serving) of lutein and would hold a promise as high-lutein staple functional foods. Bioavailability of lutein in the wholegrain bread, cookie and muffin was also investigated using fasted and fed digestion model in which food products were subjected to an in vitro simulation of human salivary, gastric and duodenal digestion, and then followed by Caco-2 monolayer absorption . The fed model resulted in much higher estimates of bioavailability of lutein and the higher fat products (cookie and muffin) resulted in higher overall bioavailability. Antioxidant capacity of the products varied among the baked products subject to product type, type of bioactive components (e.g., unbound versus bound phenolic compounds) and antioxidant assay . In the ORAC assay similar antioxidant capacities were obtained for unbound phenol extracts either from fortified or unfortified high-lutein products, while significant differences were observed for bound phenol extracts. Significant differences were also found between unbound and bound phenol extracts in their ability to scavenge ABTS radical cation. In the DPPH assay lutein-fortified products had scavenging capacities significantly higher than that of the unfortified ones. In general, the bound phenolic extracts contribute significantly higher than the unbound phenol extracts to the antioxidant capacity. Only the DPPH test showed the contribution of lutein to the antioxidant capacity. The baking process was found to increase free phenolic acids in the three products, while bound phenolic acids was decreased in bread and slightly changed in cookie and muffin products . Though the effect of baking appeared to be dependent on type of baked product, type of phenolic, recipe and baking conditions, the wholegrain products should be considered good sources of phenolic antioxidants.
Corn products are also rich in carotenoids primarily lutein and zeaxanthin and would be potential candidates for making cereal-based functional foods if high carotenoids varieties are chosen for processing. Lutein, zeaxanthin and other carotenoids in processed corn including canned corn, corn meal, corn flour and corn flake were found to vary between the products and between brands of the same product, but variations between lots of the same brand was small . Among five corn types including white, yellow, high-carotenoid, blue and red corns, lutein content was highest in yellow corn (406 µg/100 g) and lowest in blue and white corns (5.2 and 5.7 µg/100 g, respectively) . Lime-cooking significantly decreased lutein content in yellow, red and high-carotenoid corns. Further processing into tortillas and tortilla chips did not significantly affect lutein content except for yellow corn chips. The contents of lutein and zeaxanthin in corn tortilla and chips made from high-carotenoid variety are presented in Table 1.
Increasing age is the dominating factor for the onset of cataracts and AMD because of physiological and biochemical changes due to old age. Global researchers have identified lack of lutein and zeaxanthin as dietary causes in cataract and AMD related blindness. To date large scale prevalence studies provide serious data that conclude ethnicity may be the other major factors. But, age, diet and ethnicity are not the only factors for cataract and AMD. Hence prevention programs should not be solely based on these factors. Other factors such as living (geographic location) and working environment, socio-economic standing and hitherto unidentified factors should also be investigated. More research looking into the development of high-xanthophyll functional foods is essential in order to develop dietary strategies for the management of cataract and AMD in particular for elderly people.
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